Isolation and purification of chloroplastic spinach (Spinacia oleracea) sedoheptulose-1,7-bisphosphatase
نویسندگان
چکیده
منابع مشابه
Novel defensin subfamily from spinach (Spinacia oleracea).
Antimicrobial peptides (So-D1-7) were isolated from a crude cell wall preparation from spinach leaves (Spinacia oleracea cv. Matador) and, judged from their amino acid sequences, six of them (So-D2-7) represented a novel structural subfamily of plant defensins (group IV). Group-IV defensins were also functionally distinct from those of groups I-III. They were active at concentrations < 20 micro...
متن کاملCircular Dna Molecules Associated with Chloroplasts of Spinach, Spinacia Oleracea
Results of cytochemical studies (Sagan and Scher, 1961 ; Ris and Plaut, 1962 ; Kislev et al ., 1965) and of studies involving cell fractionation and buoyant density analyses (Sager and Ishida, 1963 ; Ray and Hanawalt, 1964 ; Brawerman and Eisenstadt, 1964 ; Edelman et al ., 1964 ; Wells and Birnstiel, 1969) have shown that chloroplasts of microorganisms and of higher plants contain DNA which is...
متن کاملPurification and properties of spinach leaf cytoplasmic fructose-1,6-bisphosphatase.
Cytoplasmic fructose-1,6-bisphosphatase has been purified from spinach leaves to apparent homogeneity. The enzyme is a tetramer of molecular weight about 130,000. At pH 7.5, the Km for fructose 1.6-bisphosphate was 2.5 micron, and for MgCl2 0.13 mM; the enzyme was specific for fructose 1,6-bisphosphate. Saturation with Mg2+ was achieved with lower concentrations at pH 8 than at pH 7. AMP and hi...
متن کاملIsolation and kinetic properties of acetohydroxy acid isomeroreductase from spinach (Spinacia oleracea) chloroplasts overexpressed in Escherichia coli.
Acetohydroxy acid isomeroreductase catalyses a two-step reaction, an alkyl migration and a NADPH-dependent reduction, in the assembly of the carbon skeletons of branched-chain amino acids. Detailed investigations of acetohydroxy acid isomeroreductase aimed at elucidating the biosynthetic pathway of branched-chain amino acids and at designing new inhibitors of the enzyme having herbicidal potenc...
متن کاملBiochar impact on physiological and biochemical attributes of Spinach (Spinacia oleracea L.) in nickel contaminated soil
Disastrous effect of nickel on spinach was discussed by number of authors but the effect of amendments like biochar with nickel on Spinacea oleraceaL. is not still discussed by any author of the world because biochar was used as soil amendments which play a vital role in reducing mobilization and uptake of nickel by spinach plants. As nickel contaminated plants are very harmful for th...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2410071